Interaction of deoxyribonucleic acid with silochrome modified by aminosilane

Authors

  • O. V. Markitan Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • R. B. Kozakevych Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • D. S. Kamenskyh Kukhar Institute of Bioorganic Chemistry and Petrochemistry of National Academy of Sciences of Ukraine / V. Bakul Institute for Superhard materials of National Academy of Sciences of Ukraine
  • V. A. Tertykh Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine

DOI:

https://doi.org/10.15407/hftp16.02.191

Keywords:

silochrome, chemical modification, deoxyribonucleic acid, adsorption, isotherm, adsorption models

Abstract

Materials capable of sorbing nucleic acids are in great demand for the development of sensor systems for biological purposes, analytical separation, and gene delivery. Due to their unique properties, nanoscale oxide materials are increasingly becoming the basis of research and development of new catalytic and sensor systems. Silicas are of particular interest. The stability of their physicochemical parameters, significant rate of reaching equilibrium, large surface areas, and mechanical stability contribute to the wide use of these materials for modification and immobilization on their surface various functional groups. Chemical modification of the silica surface allows one to control the strength of the adsorption interaction of substances with the surface, preserving the functionality of the adsorbate, ensuring the reversibility of the process and increasing the selectivity of materials.

The purpose of this work was to study the adsorption of deoxyribonucleic acid from an aqueous solution on the surface of silochrome modified with 3-aminopropyltriethoxysilane, depending on the pH of the medium and the concentration of the adsorbate. It has been shown that the driving force of adsorption is the electrostatic interaction between positively charged aminopropyl groups of the sorbent surface and nucleic acid anions. Adsorption of DNA in the acidic region can be carried out due to dispersion forces or hydrogen bonds between surface functional groups and highly polar secondary phosphate groups on the outer surface of the nucleic acid molecule. Based on the calculated thermodynamic parameters, the process of interaction of deoxyribonucleic acid with the surface of aminosilane modified silochrome is believed to be spontaneous and proceeds according to the ion-exchange mechanism, as a result of which sorbate molecules bind to the surface quite strongly. The implementation of electrostatic interaction between aminosylochrome and deoxyribonucleic acid allows to stabilize its structure in a certain way, creating the possibility of further interaction by intercalation with biologically active substances. This will allow the use of such organo-mineral systems as biocompatible carriers and as model structures in biotechnological, medical, and catalytic research.

References

1. Seeman N.C. Nucleic acid junctions and lattices. J. Theor. Biol. 1982. 99(2): 237. https://doi.org/10.1016/0022-5193(82)90002-9

2. Dietz H., Douglas S.M., Shih W.M. Folding DNA into Twisted and Curved Nanoscale Shapes. Science. 2009. 325(5941): 725. https://doi.org/10.1126/science.1174251

3. Han D., Pal S., Nangreave J., Deng Z., Liu Y., Yan H. DNA Origami with Complex Curvatures in Three-Dimensional Space. Science. 2011. 332(6027): 342. https://doi.org/10.1126/science.1202998

4. Rothemund P.W.K. Folding DNA to create nanoscale shapes and patterns. Nature. 2006. 440(7082): 297. https://doi.org/10.1038/nature04586

5. Linko V., Ora A., Kostiainen M.A. DNA nanostructures as smart drug-delivery vehicles and molecular devices. Trends Biotechnol. 2015. 33(10): 586. https://doi.org/10.1016/j.tibtech.2015.08.001

6. Yang H., Feng Q. Direct synthesis of pore-expanded amino-functionalized mesoporous silicas with dimethyldecylamine and the effect of expander dosage on their characterization and decolorization of sulphonated azo dyes. Microporous Mesoporous Mater. 2010. 135(1-3): 124. https://doi.org/10.1016/j.micromeso.2010.06.019

7. Yang H., Feng Q. Characterization of pore-expanded amino-functionalized mesoporous silicas directly synthesized with dimethyldecylamine and its application for decolorization of sulphonated azo dyes. J. Hazard. Mater. 2010. 180(1-3): 106. https://doi.org/10.1016/j.jhazmat.2010.03.116

8. Andrzejewska A., Krysztafkiewicz A., Jesionowski T. Treatment of textile dye wastewater using modified silica. Dyes Pigm. 2007. 75(1): 116. https://doi.org/10.1016/j.dyepig.2006.05.027

9. Joo J.B., Park J., Yi J. Preparation of polyelectrolyte-functionalized mesoporous silicas for the selective adsorption of anionic dye in an aqueous solution. J. Hazard. Mater. 2009. 168(1): 102. https://doi.org/10.1016/j.jhazmat.2009.02.015

10. Asouhidou D.D., Triantafyllidis K.S., Lazaridis N.K., Matis K.A. Adsorption of Remazol Red 3BS from aqueous solutions using APTES- and cyclodextrinmodified HMS-type mesoporous silicas. Colloids Surf., A. 2009. 346(1-3): 83. https://doi.org/10.1016/j.colsurfa.2009.05.029

11. Mahmoodi N.M., Khorramfar S., Najafi F. Amine-functionalized silica nanoparticle: Preparation, characterization and anionic dye removal ability. Desalination. 2011. 279(1-3): 61. https://doi.org/10.1016/j.desal.2011.05.059

12. Cestari A.R., Vieira E.F.S., Vieira G.S., Almeida L.E. The removal of anionic dyes from aqueous solutions in the presence of anionic surfactant using aminopropylsilica - a kinetic study. J. Hazard. Mater. 2006. 138(1): 133. https://doi.org/10.1016/j.jhazmat.2006.05.046

13. Donia A.M., Atia A.A., Al-amrani W.A., El-Nahas A.M. Effect of structural properties of acid dyes on their adsorption behaviour from aqueous solutions by amine modified silica. J. Hazard. Mater. 2009. 161(2-3): 1544. https://doi.org/10.1016/j.jhazmat.2008.05.042

14. Cestari A.R., Vieira E.F.S., Vieira G.S., Da Costa L.P, Tavares A.M.G., Loh W., Airoldi C. The removal of reactive dyes from aqueous solutions using chemically modified mesoporous silica in the presence of anionic surfactant - the temperature dependence and a thermodynamic multivariate analysis. J. Hazard. Mater. 2009. 161(1): 307. https://doi.org/10.1016/j.jhazmat.2008.03.091

15. Pavan F.A., Dias S.L.P., Lima E.C., Benvenutti E.V. Removal of Congo red from aqueous solution by anilinepropylsilica xerogel. Dyes Pigm. 2008. 76(1): 64. https://doi.org/10.1016/j.dyepig.2006.08.027

16. Fang Y., Huang X-J., Chen P-C, Xu Z-K. Polymer materials for enzyme immobilization and their application in bioreactors. BMB Reports. 2011. 44(2): 87. https://doi.org/10.5483/BMBRep.2011.44.2.87

17. Karimi M., Chaudhury I., Jianjun C., Safari M., Sadeghi R., Habibi-Rezaei M., Kokini J. Immobilization of endo-inulinase on non-porous amino functionalized silica nanoparticles. J. Mol. Catal. B: Enzym. 2014. 104: 48. https://doi.org/10.1016/j.molcatb.2014.01.025

18. Orçaire O., Buisson P., Pierre A.C. Application of silica aerogel encapsulated lipases in the synthesis of biodiesel by transesterification reactions. J. Mol. Catal. B: Enzym. 2006. 42(3-4): 106. https://doi.org/10.1016/j.molcatb.2006.08.002

19. Chen Z., Hsu F.-C., Battigelli D., Chang H.-C. Capture and release of viruses using amino-functionalized silica particles. Anal. Chim. Acta. 2006. 569(1-2): 76. https://doi.org/10.1016/j.aca.2006.03.103

20. Carré A., Lacarrière V., Birch W. Molecular interactions between DNA and an aminated glass substrate. J. Colloid Interface Sci. 2003. 260(1): 49. https://doi.org/10.1016/S0021-9797(02)00147-9

21. Balladur V.V., Theretz A., Mandrand B. Determination of the Main Forces Driving DNA Oligonucleotide Adsorption onto Aminated Silica Wafers. J. Colloid Interface Sci. 1997. 194(2): 408. https://doi.org/10.1006/jcis.1997.5123

22. Tuite E., Kelly J.M. The Interaction of Methylene Blue, Azure B, and Thionine with DNA: Formation of Complexes with Polynucleotides and Mononucleotides as Model Systems. Biopolymers. 1995. 35(5): 419. https://doi.org/10.1002/bip.360350502

23. Aslanoglu M. Electrochemical and spectroscopic studies of the interaction of proflavine with DNA. Anal. Sci. 2006. 22(3): 439. https://doi.org/10.2116/analsci.22.439

24. Bereznyak E.G., Gladkovskaya N.A., Khrebtova A.S., Dukhopelnikov E.V., Zinchenko A.V. Peculiarities of DNA-Proflavine Binding under Different Concentration Ratios. Biophysics. 2009. 54(5): 574. https://doi.org/10.1134/S0006350909050030

25. Thommes M., Kaneko K., Neimark A.V., Olivier J.P., Rodriguez-Reinoso F., Rouquerol J., Sing K.S.W. Physisorption of gases, with special reference to the evaluation of surface area and pore size distribution (IUPAC Technical Report). Pure Appl. Chem. 2015. 87(9-10): 1051. https://doi.org/10.1515/pac-2014-1117

26. Vlasova N.N. Adsorption of amino acids on the surface of cerium dioxide. Colloid. J. 2016. 78(6): 700. [in Russian]. https://doi.org/10.1134/S1061933X16060181

27. Saenger W. Principles of nucleic acids structure. (Advansed texts in chemistry series. New York: Springer-Verlag, 1983). https://doi.org/10.1007/978-1-4612-5190-3

28. Lehninger A. Biochemistry: the molecular basis of cell structure and function. (New York: Worth Publishers, Inc., 1972).

29. Thaplyal P., Bevilacqua P.C. Chapter Nine - Experimental Approaches for Measuring pKa's in RNA and DNA. Methods Enzymol. 2014. 549: 189. https://doi.org/10.1016/B978-0-12-801122-5.00009-X

30. Suzuki H., Amano T., Toyooka T. Preparation of DNA-adsorbed TiO2 particles with high performance for purification of chemical pollutants. Environ. Sci. Technol. 2008. 42(21): 8076. https://doi.org/10.1021/es800948d

31. Abu-Salah Kh.M., Ansari A.A., Alrokayan S.A. DNA-Based Applications in Nanobiotechnology. J. Biomed. Biotechnol. 2010. 2010: 715295. https://doi.org/10.1155/2010/715295

32. Sun Y., Kiang C-H. DNA-based artificial nanostructures: Fabrication, properties, and applications. In: Handbook of Nanostructured Biomaterials and Their Applications in Nanobiotechnology. (American Scientific Publishers, 2005).

33. Pautler R., Kelly E.Y., Huang P-J. J., Cao J., Liu B., Liu J. Attaching DNA to nanoceria: regulating oxidase activity and fluorescence quenching. ACS Appl. Mater. Interfaces. 2013. 5(15): 6820. https://doi.org/10.1021/am4018863

34. Langmuir I. The constitution and fundamental properties of solids and liquids. J. Am. Chem. Soc. 1916. 38(11): 2221. https://doi.org/10.1021/ja02268a002

35. Freundlich H.M.F. Űber die Adsorption in Lösungen. Z. Phys. Chem. 1906. 57: 385. https://doi.org/10.1515/zpch-1907-5723

36. Dubinin M.M., Radushkevich L.V. On the equation of the characteristic curve for active coals. Proceedings of the USSR Academy of Sciences. 1947. 55: 331.

37. Hall K.R., Eagleton L.C., Acrivos A., Vermeulen T. Pore and solid diffusion kinetics in fixed-bed adsorption under constant pattern conditions. Ind. Eng. Chem. Fundam. 1966. 5(2): 212. https://doi.org/10.1021/i160018a011

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Published

01.06.2025

How to Cite

(1)
Markitan, O. V.; Kozakevych, R. B.; Kamenskyh, D. S.; Tertykh, V. A. Interaction of Deoxyribonucleic Acid With Silochrome Modified by Aminosilane. Him. Fiz. Tehnol. Poverhni 2025, 16, 191-203.

Issue

Vol. 16 No. 2 (2025): Chemistry, Physics and Technology of Surface / Himia, Fizika ta Tehnologia Poverhni

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