Chemistry, Physics and Technology of Surface, 2012, 3 (2), 155-165.

The Peculiarities of Hydration of Hyaluronic Acid According to 1H NMR Spectroscopy



V. V. Turov, A. P. Ugnivenko, A. P. Golovan, V. N. Barvinchenko, V. M. Gun'ko

Abstract


The hydratation of hyaluronic acid in air, frozen aqueous solutions, and weakly polar solvent CDCl3 has been studied by low-temperature 1H NMR spectroscopy. Thermodynamic parameters of the strongly and weakly bounded water as well as interfacial energy of polymer-water system have been specified. It has been found that environment of CDCl3 produces a differentiating effect on bounded water in case of low hydration. Four signals of strongly associated water and one signal of weakly associated water have been detected separately in the spectra. A joint water system is formed with increasing hydration. The maximum value of interfacial energy of polymer-water in the aqueous solution is observed at 2% hyaluronic acid what corresponds to the beginning of gel structure formation.

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Copyright (©) 2012 V. V. Turov, A. P. Ugnivenko, A. P. Golovan, V. N. Barvinchenko, V. M. Gun'ko

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