Email the author Encapsulation of cellular suspensions of lactic bacteria with silica
DOI: https://doi.org/10.15407/hftp12.01.058
Abstract
A promising direction for long-term storage of cells at relatively high temperatures may be their encapsulation with nanoscale biologically inert materials capable of creating a shell around microdroplets of a cell suspension, which, on the one hand, provides the possibility of gas exchange between the suspension and the external environment, and on the other hand, inhibits the processes of cell life, so transferring them to a state close to suspended animation. The method of low-temperature 1H NMR spectroscopy was used to study the process of hydration of lactobacilli, the effect of a weakly polar organic medium on it and the encapsulation of cells with silica. The aim of this work was to study the hydration of cell suspensions and the viability of lactic acid bacteria cells encapsulated with silica and the penetration possibility of such an active substance as trifluoroacetic acid into them. As a result of the studies carried out, it has been shown that the spectral parameters of water in concentrated cell suspensions of lactic acid bacteria strongly depend on the concentration of the suspension, which is probably associated with the possibility of the formation of a stable cell gel, which can be encapsulated by silica particles without its destruction in both air and a chloroform medium with addition of trifluoroacetic acid. The radial distribution curves of non-freezing water clusters have two maxima corresponding to R = 2 and 20–100 nm. The contribution to the distribution of the second maximum increases with increasing water concentration.
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References
1. Franks F. Biophysics and biochemistry at low temperature. (Cambridge: University Press, 1985).
2. Kuleshova L. G., Kovalenko I.F. Theoretical prediction of optimal cooling rates of cell suspensions. Bulletin of the Kharkov National University. Biophysical Bulletin. 2008. 20(1): 56. [in Russian].
3. Osetskii A.I., Kirilyuk A.L., Gurina T.M. On the possible mechanism of damage to cryopreserved biological objects due to plastic pressure relaxation in closed liquid-phase inclusions. Problems of Cryobiology. 2007. 17(3): 272. [in Russian].
4. Krivokharchenko A.S., Serobyan G.A., Sadovnikov V.B. Cryoembryobanks - promising technology for the conservation of laboratory, domestic and wild animal genetic resources for practical use. In: IV International Conference and Discussion Scientific Club. Ukraine, Crimea, Yalta-Gurzuf: "IT+ME'98", 1998. P. 380. [in Russian].
5. Kirilenko M.A., Kuznetsov O.Yu., Dmitrieva Zh.M. The effect of cryopreservation on the survival of a complex of lactic acid bacterial autostrains during storage and biotechnological scaling processes. Yu.A. Ovchinnikova Herald of biotechnology and physical and chemical biology. 2019. 15(2): 5. [in Russian].
6. Hofmann N., Bernemann I., Pogozhi D., Glasmaher B. Development of system optimization of cryopreservation protocols for cell suspensions. Problems of Cryobiology. 2011. 21(4): 353. [in Russian].
7. Zvyagintsev D.G. Interaction of microorganisms with solid surfaces. (Moscow: Publishing house of the MSU, 1973). [in Russian].
8. Tsyrenov V.Zh. The basics of biotechnology: the cultivation of human and animal cells. (SSSTU Ulan-Ude, 2005). [in Russian].
9. Lapage S.P., Shelton J.E., Mitchell T.G., Mackenzie A.R. Culture collections and the preservation of bacteria. In Methods in Microbiology. (London: Academic Press, 1970). P. 135. https://doi.org/10.1016/S0580-9517(08)70540-3
10. Malik K.A., Claus D. Bacterial culture collection: Their importance to biotechnology and microbiology. Biotechnology and Genetic Engineering Reviews. 1987. 5: 137. https://doi.org/10.1080/02648725.1987.10647837
11. Uzunova-Doneva T., Donev T. Anabiosis and conservation of microorganisms. Journal of Culture Collections. 2005. 4: 17.
12. Sidyakina T.M. Preservation of microorganisms. (Pushchino: ONTI NCBI, 1985). [in Russian].
13. Fakhrullin R.F., Minullina R.T. Hybrid Cellular−Inorganic Core−Shell Microparticles: Encapsulation of Individual Living Cells in Calcium Carbonate Microshells. Langmuir. 2009. 25(12): 6617. https://doi.org/10.1021/la901395z
14. Gun'ko V.M., Turov V.V., Gorbik P.P. Water at the interface. (Kyiv: Naukova Dumka, 2009). [in Russian].
15. Gun'ko V.M., Turov V.V. Nuclear Magnetic Resonance Studies of Interfacial Phenomena. (New York: Taylor & Francis, 2013). https://doi.org/10.1201/b14202
16. Turov V.V., Gun'ko V.M. Clustered water and ways to use it. (Kyiv: Naukova dumka, 2011). [in Russian]
17. Gun'ko V.M., Turov V.V., Bogatyrev V.M., Zarko V.I., Leboda R., Goncharuk E.V., Novza A.A., Turov A.V., Chuiko A.A. Unusual Properties of Water at Hydrophilic/Hydrophobic Interfaces. Adv. Colloid Interface Sci. 2005. 118(1-3): 125. https://doi.org/10.1016/j.cis.2005.07.003
18. Aksnes D.W., Kimtys L. Characterization of mesoporous solids by 1H NMR. Magnetic Resonance in Colloid and Interface Science. 2004. 25: 146. https://doi.org/10.1016/j.ssnmr.2003.03.001
19. Petrov O.V., Furo I. NMR cryoporometry: Principles, application and potential. Progress in Nuclear Magnetic Resonance Spectroscopy. 2009. 54(2): 97. https://doi.org/10.1016/j.pnmrs.2008.06.001
20. Frolov Yu.G. The course of colloid chemistry. Surface phenomena and disperse systems. (Moscow: Chemistry, 1982). [in Russian].
21. Pople J.A., Schneider W.G., Bernstein H.J. High-Resolution Nuclear Magnetic Resonance. (New York-Toronto-London: McGraw-Hill Book Company, JNC, 1959).
DOI: https://doi.org/10.15407/hftp12.01.058
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