The Peculiarities of Hydration of Hyaluronic Acid According to <sup>1</sup>H NMR Spectroscopy

Authors

  • V. V. Turov Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • A. P. Ugnivenko Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • A. P. Golovan Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • V. N. Barvinchenko Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • V. M. Gun'ko Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine

Abstract

The hydratation of hyaluronic acid in air, frozen aqueous solutions, and weakly polar solvent CDCl3 has been studied by low-temperature 1H NMR spectroscopy. Thermodynamic parameters of the strongly and weakly bounded water as well as interfacial energy of polymer-water system have been specified. It has been found that environment of CDCl3 produces a differentiating effect on bounded water in case of low hydration. Four signals of strongly associated water and one signal of weakly associated water have been detected separately in the spectra. A joint water system is formed with increasing hydration. The maximum value of interfacial energy of polymer-water in the aqueous solution is observed at 2% hyaluronic acid what corresponds to the beginning of gel structure formation.

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V.3, N2

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Turov, V. V.; Ugnivenko, A. P.; Golovan, A. P.; Barvinchenko, V. N.; Gun’ko, V. M. The Peculiarities of Hydration of Hyaluronic Acid According to <sup>1< Sup>H NMR Spectroscopy. Him. Fiz. Tehnol. Poverhni 2012, 3, 155-165.

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Vol. 3 No. 2 (2012): Chemistry, Physics and Technology of Surface / Himia, Fizika ta Tehnologia Poverhni

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