The Behaviour of Intracellular Water in Yeast Cells Affected by Organic Solvents

Authors

  • V. M. Gun'ko Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • T. V. Krupska Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • V. M. Barvinchenko Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine
  • V. V. Turov Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine

Abstract

Yeast Saccharomyces cerevisiae cells dry (containing approximately 7 wt.% of water) and partially rehydrated (10 wt.% addition of water) and with added organic solvents (10 wt.% or 2–3 g per gram of dry cells) nonpolar (C6H6, CCl4) or polar (CD3CN, (CH3)2CO, (CD3)2CO, (CH3)2SO, (CD3)2SO) were studied using 1H NMR spectroscopy with layer-by-layer freezing-out of bound and bulk liquids at 200–273 K, TG/DTA and FTIR spectroscopy methods. Nonpolar solvents displace a portion of water into narrower ‘pores’ in contrast to polar solvents which displace water into larger ‘mesopores’ in the intracellular space. This structural feature of intracellular water is accompanied by its energetic differentiation, and four types of intracellular water are found: weakly (chemical shift dH = 1–2 ppm) and strongly associated (dH = 3–5 ppm) waters which can be strongly (DG < -0.8 kJ/mol) or weakly (DG > -0.8 kJ/mol) bound to intracellular functionalities.

References

Crowe J.H., Hoekstra F.A., Crowe L.M. Annu. Rev. Physiol. 1992. 54. 579. DOI: 10.1146/annurev.ph.54.030192.003051.

Dilks T.J.K., Proctor M.C.F. J. Bryol. 1974. 8. 97.

Leopold A.C. (Ed.) Membrane, metabolism and dry organisms. Comstock Pub. Assoc., 1986. 188–209.

Tunnacliffe A., de Castro A.G., Manzanera M. Cryobiology. 2001. 43(2). 124. doi 10.1006/cryo.2001.2356.

Becker M.E., Rapoport A.I., Kalakutsi L.В. Detention of the cell vitality. Riga: Zinatne, 1987. (in Russian).

Cameron R.E., Morelli F.A. Antarctic J. U.S. 1974. 9. 113.

Gilichinsky D. (Ed.) Viable microorganisms in permafrost. Russian Acad. Sci.: Pushchino, 1994.

Gilichinsky D.A., Vorobyova E.A., Erokhina L.G., Fyordorov-Dayvdova D.G., Chaikovskay N.R. Adv. Space Res. 1992. 12(5). 225. doi 10.1016/0273-1177(92)90180-6.

Jones P.G., VanBogelen R.A., Neidhardt F.C. J. Bacteriol. 1987. 169(5). 2092. PMC212099.

Jones P.G., Inouye M. Mol. Microbiol. 1994. 11(5). 811.

Gounot A.-M. J. Appl. Bacteriol. 1991. 71(5). 386.

Steponkus P.L., Uemura M., Webb M.S. Curr. Topics Plant Physiol. 1993. 10. 37.

Steponkus P.L., Uemura M., Joseph R.A., Gilmour S.J., Thomashow M.F. Proc. Natl. Acad. Sci. USA. 1998. 95. 14570. doi: 10.1073/pnas.95.24.14570.

Strauss G., Hauser H. Proc. Natl. Acad. Sci. USA. 1986. 83(8). 2422.

Anchordoguy T.J., Rudolph A.S., Carpenter J.F., Crowe J.H. Cryobiology. 1987. 24(4). 324.

Nanjo T., Kobayashi M., Yoshiba Y., Kakubari Y, Yamaguchi-Shinozaki K., Shinozaki K. FEBS Lett. 1999. 461(3). 205.

Antikainen M., Griffith M. Physiol. Plant. 1997. 99(3). 423.

Armitage W.J., Juss B.K. Cryo-Lett. 1996. 17(4). 213.

Karlsson J.O.M. Cryobiology. 2001. 42(3). 154.

Hubalek Z. Cryobiology. 2003. 46(3). 205. DOI: 10.1111/j.1365-2672.1991.tb03806.x.

Ashwood-Smith M.J., Farrant J. (Eds.) Low Temperature preservation in medicine and biology. Pitman, Kent, England, 1980.

Wang J.-H. Cryobiology. 2000. 41(1). 1.

Tao D., Li P.H. J. Theor. Biol. 1986. 123(3). 305.

Uzunova-Doneva T., Donev T. J. Culture Collect. 2005. 4(1). 17.

Turov V.V., Gun’ko V.M., Bogatyrev V.M., Zarko VI, Gorbik SP, Pakhlov EM, Leboda R, Shulga OV, Chuiko AA. J. Colloid Interface Sci. 2005. 283(2). 329.

Gun’ko V.M., Turov V.V., Bogatyrev V.M. et al. Adv. Colloid Interface Sci. 2005. 118(1–3). 125.

Surface Chemistry in Biomedical and Environmental Science, NATO Science Series II: Mathematics, Physics and Chemistry / Eds. J.P. Blitz and V.M. Gun'ko. Dordrecht: Springer, 2006.

Turov V.V., Gun'ko V.M., Tsapko M.D. et al. Appl. Surf. Sci. 2004. 229(1–4). 197.

Pople J.A., Schneider W.G., Bernstein H.J. High-resolution nuclear magnetic resonance. New York: McGraw-Hill Book Company, 1959.

Hindman J.C. J. Chem. Phys. 1966. 44(12). 4582.

Doremieux-Morin C., Heeribout L., Dumousseaux C. et al. J. Am. Chem. Soc. 1996. 118(51). 13040.

Gun’ko V.M., Turov V.V., Shpilko A.P. et al. Colloids Surf. B. 2006. 53(1). 29.

Turov V.V., Gun’ko V.M., Zarko V.I. et al. Colloids Surf. B. 2006. 48(2). 167.

Bergna H.E. (Ed.) Colloidal silica: fundamentals and applications. Taylor & Francis LLC, Salisbury, 2005. 499–530.

Gun’ko V.M., Turov V.V. Langmuir. 1999. 15(19). 6405.

Handbook of thermodynamic properties of individual substances. Ed. V.P. Glushko. Moscow: Nauka, 1978. (in Russian).

Strange J.H., Rahman M., Smith E.G. Phys. Rev. Lett. 1993. 71(21). 3589.

Strange J.H., Mitchell J., Webber J.B.W. Magn. Reson. Imag. 2003. 21(3–4). 221.

Gallegos D.P., Munn K., Smith D.M., Stermer D.L. J. Colloid Interface Sci. 1986. 119(1). 127.

Aksnes D.W., Kimtys L. Solid State Nuclear Mag. Res. 2004. 25(1–3). 146.

Gun’ko V.M., Turov V.V., Leboda R. et al. Langmuir. 2007. 23(6). 3184.

Gun’ko V.M., Turov V.V., Zarko V.I. et al. J. Colloid Interface Sci. 2007. 308(1). 142.

Gun’ko V.M., Turov V.V., Leboda R. et al. Appl. Surf. Sci. 2007. 253(13). 5640.

Provencher S.W. Comp. Phys. Comm. 1982. 27(3). 213.

Gun’ko V.M., Turov V.V., Leboda R. et al. Adsorption. 2005. 11(1). 163.

Turov V.V., Gun’ko V.M., Leboda R. et al. J. Colloid Interface Sci. 2002. 253(1). 23.

Gun'ko V.M., Turov V.V., Turov A.V. et al. Central Europ. J. Chem. 2007. 5. 420.

Physicochemistry of Nanomaterials and Supramolecular Structures. Eds. A.P. Shpak and P.P. Gorbik. Kiev: Naukova Dumka, 2007. (in Russian).

Chaplin M. Water structure and science. http://www.lsbu.ac.uk/water/. 2012.

Gun'ko V.M., Turov V.V., Gorbik P.P. Water at the Interfaces. Kiev: Naukova Dumka, 2009. (in Russian).

Turov V.V., Gun'ko V.M. Clustered Water and Ways of its Applications. Kiev: Naukova Dumka, 2011. (in Russian).

Downloads

How to Cite

(1)
Gun’ko, V. M.; Krupska, T. V.; Barvinchenko, V. M.; Turov, V. V. The Behaviour of Intracellular Water in Yeast Cells Affected by Organic Solvents. Him. Fiz. Tehnol. Poverhni 2012, 3, 359-374.

Issue

Vol. 3 No. 4 (2012): Chemistry, Physics and Technology of Surface / Himia, Fizika ta Tehnologia Poverhni

Section

Articles

License

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.